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Heterologous Expression of Mycobacterial Esx Complexes in Escherichia coli for Structural Studies Is Facilitated by the Use of Maltose Binding Protein Fusions

The expression of heteroligomeric protein complexes for structural studies often requires a special coexpression strategy. The reason is that the solubility and proper folding of each subunit of the complex requires physical association with other subunits of the complex. The genomes of pathogenic m...

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Detalhes bibliográficos
Main Authors: Arbing, Mark A., Chan, Sum, Harris, Liam, Kuo, Emmeline, Zhou, Tina T., Ahn, Christine J., Nguyen, Lin, He, Qixin, Lu, Jamie, Menchavez, Phuong T., Shin, Annie, Holton, Thomas, Sawaya, Michael R., Cascio, Duilio, Eisenberg, David
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3843698/
https://ncbi.nlm.nih.gov/pubmed/24312350
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0081753
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