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Probing impact of active site residue mutations on stability and activity of Neisseria polysaccharea amylosucrase

The amylosucrase from Neisseria polysaccharea is a transglucosidase from the GH13 family of glycoside-hydrolases that naturally catalyzes the synthesis of α-glucans from the widely available donor sucrose. Interestingly, natural molecular evolution has modeled a dense hydrogen bond network at subsit...

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Main Authors: Daudé, David, Topham, Christopher M, Remaud-Siméon, Magali, André, Isabelle
Format: Artigo
Jezik:Inglês
Izdano: Blackwell Publishing Ltd 2013
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3843629/
https://ncbi.nlm.nih.gov/pubmed/24115119
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.2375
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