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Escherichia coli polymerase I can use O2-methyldeoxythymidine or O4-methyldeoxythymidine in place of deoxythymidine in primed poly(dA-dT).poly(dA-dT) synthesis.

O2-and O4-alkyldeoxythymidine are among the four O-alkyl base-modified derivatives produced by the reaction of N-nitroso alkylating agents with nucleic acids in vitro and in vivo. We find that both O2- and O4-methyl-dTTP can substitute for dTTP in alternating poly(dA-dT)-primed DNA synthesis. Up to...

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Bibliografski detalji
Izdano u:Proc Natl Acad Sci U S A
Glavni autori: Singer, B, Sági, J, Kuśmierek, J T
Format: Artigo
Jezik:Inglês
Izdano: National Academy of Sciences 1983
Teme:
Online pristup:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC384151/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6348776/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.80.16.4884
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