Cloning of human purine-nucleoside phosphorylase cDNA sequences by complementation in Escherichia coli.
We have obtained cDNA clones that contain the entire coding region of the human purine-nucleoside phosphorylase (PNP; EC 2.4.2.1) mRNA. The cDNA sequences were generated by reverse transcription of PNP-enriched mRNA obtained by immunoadsorption of HeLa cell polyribosomes with monospecific antibody t...
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| Pubblicato in: | Proc Natl Acad Sci U S A |
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| Autori principali: | , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
National Academy of Sciences
1983
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC384021/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6410388/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.80.14.4281 |
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