Cloning of human purine-nucleoside phosphorylase cDNA sequences by complementation in Escherichia coli.

We have obtained cDNA clones that contain the entire coding region of the human purine-nucleoside phosphorylase (PNP; EC 2.4.2.1) mRNA. The cDNA sequences were generated by reverse transcription of PNP-enriched mRNA obtained by immunoadsorption of HeLa cell polyribosomes with monospecific antibody t...

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Veröffentlicht in:	Proc Natl Acad Sci U S A
Hauptverfasser:	Goddard, J M, Caput, D, Williams, S R, Martin, D W
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	National Academy of Sciences 1983
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC384021/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6410388/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.80.14.4281
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