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Cloning of human purine-nucleoside phosphorylase cDNA sequences by complementation in Escherichia coli.

We have obtained cDNA clones that contain the entire coding region of the human purine-nucleoside phosphorylase (PNP; EC 2.4.2.1) mRNA. The cDNA sequences were generated by reverse transcription of PNP-enriched mRNA obtained by immunoadsorption of HeLa cell polyribosomes with monospecific antibody t...

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Autors principals: Goddard, J M, Caput, D, Williams, S R, Martin, D W
Format: Artigo
Idioma:Inglês
Publicat: 1983
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC384021/
https://ncbi.nlm.nih.gov/pubmed/6410388
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