Microinjection of fluorescently labeled α-actinin into living fibroblasts
α-Actinin from chicken gizzard labeled with tetramethylrhodamine isothiocyanate has been incorporated into living fibroblast cells by microinjection. Fluorescent labeling of α-actinin was carried out such that the conjugated protein was functional in vitro as shown by its ability to bind to F-actin....
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| Publicado en: | Proc Natl Acad Sci U S A |
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| Autor Principal: | |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
National Academy of Sciences
1979
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC383957/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/291056/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.76.8.3967 |
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