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Microinjection of fluorescently labeled α-actinin into living fibroblasts

α-Actinin from chicken gizzard labeled with tetramethylrhodamine isothiocyanate has been incorporated into living fibroblast cells by microinjection. Fluorescent labeling of α-actinin was carried out such that the conjugated protein was functional in vitro as shown by its ability to bind to F-actin....

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Bibliographic Details
Main Author: Feramisco, James R.
Format: Artigo
Language:Inglês
Published: 1979
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC383957/
https://ncbi.nlm.nih.gov/pubmed/291056
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