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Microinjection of fluorescently labeled α-actinin into living fibroblasts

α-Actinin from chicken gizzard labeled with tetramethylrhodamine isothiocyanate has been incorporated into living fibroblast cells by microinjection. Fluorescent labeling of α-actinin was carried out such that the conjugated protein was functional in vitro as shown by its ability to bind to F-actin....

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:Proc Natl Acad Sci U S A
Prif Awdur: Feramisco, James R.
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: National Academy of Sciences 1979
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC383957/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/291056/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.76.8.3967
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