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Native Chemical Ligation at Asx-Cys, Glx-Cys: Chemical Synthesis and High Resolution X-ray Structure of ShK Toxin by Racemic Protein Crystallography

We have re-examined the utility of native chemical ligation at −Gln/Glu-Cys− [Glx-Cys] and −Asn/Asp-Cys− [Asx-Cys] sites. Using the improved thioaryl catalyst 4-mercaptophenylacetic acid (MPAA), native chemical ligation could be performed at −Gln-Cys− and Asn-Cys− sites without side reactions. After...

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Bibliografische gegevens
Hoofdauteurs: Dang, Bobo, Kubota, Tomoya, Mandal, Kalyaneswar, Bezanilla, Francisco, Kent, Stephen B. H.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 2013
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3838204/
https://ncbi.nlm.nih.gov/pubmed/23919482
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja4046795
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