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Stromal Cell-Independent B-Cell Development In Vitro: Generation and Recovery of Autoreactive Clones

We describe and characterize a stromal-cell independent culture system that efficiently supports pro-B cell to IgM(+) B-cell development with near normal levels of IgH and Igκ diversity. Pro-B cells present in non-adherent bone marrow cells proliferate in the presence of IL-7 and subsequent to the r...

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Detalhes bibliográficos
Main Authors: Holl, T. Matt, Haynes, Barton F., Kelsoe, Garnett
Formato: Artigo
Idioma:Inglês
Publicado em: 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3835306/
https://ncbi.nlm.nih.gov/pubmed/20109461
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jim.2010.01.007
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