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Antigen-specific identification and cloning of hybridomas with a fluorescence-activated cell sorter.
Myeloma-spleen cell hybrids (hybridomas) producing antibody to mouse immunoglobulin allotypes have been labeled with fluorescent microspheres coupled with myeloma protein antigens. The ratio of specific to nonspecific microsphere binding by viable hybridoma cells was about 100:1. By using a modified...
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| Publicado no: | Proc Natl Acad Sci U S A |
|---|---|
| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1979
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC383513/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/287037/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.76.4.1962 |
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