Antigen-specific identification and cloning of hybridomas with a fluorescence-activated cell sorter.

Myeloma-spleen cell hybrids (hybridomas) producing antibody to mouse immunoglobulin allotypes have been labeled with fluorescent microspheres coupled with myeloma protein antigens. The ratio of specific to nonspecific microsphere binding by viable hybridoma cells was about 100:1. By using a modified...

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Bibliografiska uppgifter
Huvudupphovsmän: Parks, D R, Bryan, V M, Oi, V T, Herzenberg, L A
Materialtyp: Artigo
Språk:Inglês
Publicerad: 1979
Ämnen:
Research Article
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC383513/
https://ncbi.nlm.nih.gov/pubmed/287037
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