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Strategies to construct null and conditional null Trypanosoma brucei mutants using Cre-recombinase and loxP
We describe two gene-knockout (KO) strategies in Trypanosoma brucei using Cre recombinase and loxP sites. Due to the limited number of selection markers for T. brucei, it has been difficult to generate a mutant with two genes knocked out and impractical to simultaneously knockout more than two genes...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3830529/ https://ncbi.nlm.nih.gov/pubmed/23954366 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.molbiopara.2013.08.001 |
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