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Robust red FRET sensors using self-associating fluorescent domains

Elucidation of sub-cellular signaling networks by multiparameter imaging is hindered by a lack of sensitive FRET pairs spectrally compatible with the classic CFP/YFP pair. Here we present a generic strategy to enhance the traditionally poor sensitivity of red FRET sensors by developing self-associat...

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Main Authors: Lindenburg, Laurens H., Hessels, Anne M., Ebberink, Eduard H.T.M., Arts, Remco, Merkx, Maarten
Format: Artigo
Jezik:Inglês
Izdano: 2013
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3826083/
https://ncbi.nlm.nih.gov/pubmed/23962156
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/cb400427b
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