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Robust red FRET sensors using self-associating fluorescent domains

Elucidation of sub-cellular signaling networks by multiparameter imaging is hindered by a lack of sensitive FRET pairs spectrally compatible with the classic CFP/YFP pair. Here we present a generic strategy to enhance the traditionally poor sensitivity of red FRET sensors by developing self-associat...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Lindenburg, Laurens H., Hessels, Anne M., Ebberink, Eduard H.T.M., Arts, Remco, Merkx, Maarten
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2013
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3826083/
https://ncbi.nlm.nih.gov/pubmed/23962156
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/cb400427b
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