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Heritable genome editing in C. elegans via a CRISPR-Cas9 system
CRISPR-Cas systems have been used with single-guide RNAs for accurate gene disruption and conversion in multiple biological systems. Here we report the use of the endonuclease Cas9 to target genomic sequences in the C. elegans germline, utilizing single-guide RNAs that are expressed from a U6 small...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3822328/ https://ncbi.nlm.nih.gov/pubmed/23817069 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.2532 |
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