Enzyme activity effects of N-terminal His-tag attached to catalytic sub-unit of phosphoinositide-3-kinase

NTT (N-terminal tags) on the catalytic (p110) sub-unit of PI 3-K (phosphoinositol 3-kinase) have previously been shown to increase cell signalling and oncogenic transformation. Here we test the impact of an NT (N-terminal) His-tag on in vitro lipid and protein kinase activity of all class-1 PI 3-K i...

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Detalhes bibliográficos
Main Authors: Dickson, James M. J., Lee, Woo-Jeong, Shepherd, Peter R., Buchanan, Christina M.
Formato: Artigo
Idioma:Inglês
Publicado em: Portland Press Ltd. 2013
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Original Paper
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3817851/
https://ncbi.nlm.nih.gov/pubmed/23968392
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1042/BSR20130075
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