A Single Mutation in a Regulatory Protein Produces Evolvable Allosterically Regulated Catalyst of Unnatural Reaction

[Image: see text] It only takes one mutation: a strategically placed single mutation in a non-enzymatic protein scaffold produced AlleyCat, a small, allosterically regulated catalyst of Kemp elimination. In only 7 rounds of directed evolution enzymatic efficiency of the original 74 amino acid residu...

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Auteurs principaux: Moroz, Olesia V., Moroz, Yurii S., Wu, Yibing, Olsen, Alissa B., Cheng, Hong, Mack, Korrie L., McLaughlin, Jaclyn M., Raymond, Elizabeth A., Zhezherya, Krystyna, Roder, Heinrich, Korendovych, Ivan V.
Format: Artigo
Langue:Inglês
Publié: 2013
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Article
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3817844/
https://ncbi.nlm.nih.gov/pubmed/23630096
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/anie.201302339
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