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Inter‐conversion of catalytic abilities in a bifunctional carboxyl/feruloyl‐esterase from earthworm gut metagenome
Carboxyl esterases (CE) exhibit various reaction specificities despite of their overall structural similarity. In present study we have exploited functional metagenomics, saturation mutagenesis and experimental protein evolution to explore residues that have a significant role in substrate discrimin...
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| Auteurs principaux: | , , , , , , , , , , |
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| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Blackwell Publishing Ltd
2010
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3815946/ https://ncbi.nlm.nih.gov/pubmed/21255305 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/j.1751-7915.2009.00135.x |
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