Inter‐conversion of catalytic abilities in a bifunctional carboxyl/feruloyl‐esterase from earthworm gut metagenome

Carboxyl esterases (CE) exhibit various reaction specificities despite of their overall structural similarity. In present study we have exploited functional metagenomics, saturation mutagenesis and experimental protein evolution to explore residues that have a significant role in substrate discrimin...

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Detalhes bibliográficos
Main Authors: Vieites, José María, Ghazi, Azam, Beloqui, Ana, Polaina, Julio, Andreu, José M., Golyshina, Olga V., Nechitaylo, Taras Y., Waliczek, Agnes, Yakimov, Michail M., Golyshin, Peter N., Ferrer, Manuel
Formato: Artigo
Idioma:Inglês
Publicado em: Blackwell Publishing Ltd 2010
Assuntos:
Research Articles
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3815946/
https://ncbi.nlm.nih.gov/pubmed/21255305
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/j.1751-7915.2009.00135.x
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