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Highly Improved Gene Targeting by Germline-Specific Cas9 Expression in Drosophila
We report a simple yet extremely efficient platform for systematic gene targeting by the RNA-guided endonuclease Cas9 in Drosophila. The system comprises two transgenic strains: one expressing Cas9 protein from the germline-specific nanos promoter and the other ubiquitously expressing a custom guide...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Genetics Society of America
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3813859/ https://ncbi.nlm.nih.gov/pubmed/24002648 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/genetics.113.156737 |
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