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Targeted Heritable Mutation and Gene Conversion by Cas9-CRISPR in Caenorhabditis elegans
We have achieved targeted heritable genome modification in Caenorhabditis elegans by injecting mRNA of the nuclease Cas9 and Cas9 guide RNAs. This system rapidly creates precise genomic changes, including knockouts and transgene-instructed gene conversion.
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Genetics Society of America
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3813846/ https://ncbi.nlm.nih.gov/pubmed/23979578 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/genetics.113.155754 |
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