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Single residue substitutions that change the gating properties of a mechanosensitive channel in Escherichia coli.

MscL is a channel that opens a large pore in the Escherichia coli cytoplasmic membrane in response to mechanical stress. Previously, we highly enriched the MscL protein by using patch clamp as a functional assay and cloned the corresponding gene. The predicted protein contains a largely hydrophobic...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Blount, P, Sukharev, S I, Schroeder, M J, Nagle, S K, Kung, C
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1996
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC38113/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8876191/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.93.21.11652
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