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Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of SpyCEP, a candidate antigen for a vaccine against Streptococcus pyogenes

Streptococcus pyogenes (Group A streptococcus; GAS) is an important human pathogen against which an effective vaccine does not yet exist. The S. pyogenes protein SpyCEP (S. pyogenes cell-envelope proteinase) is a surface-exposed subtilisin-like serine protease of 1647 amino acids. In addition to its...

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Bibliografiske detaljer
Main Authors: Abate, Francesca, Malito, Enrico, Falugi, Fabiana, Margarit Y Ros, Immaculada, Bottomley, Matthew James
Format: Artigo
Sprog:Inglês
Udgivet: International Union of Crystallography 2013
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3792666/
https://ncbi.nlm.nih.gov/pubmed/24100558
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309113024871
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