The Origin of Biased Sequence Depth in Sequence-Independent Nucleic Acid Amplification and Optimization for Efficient Massive Parallel Sequencing

Sequence Independent Single Primer Amplification is one of the most widely used random amplification approaches in virology for sequencing template preparation. This technique relies on oligonucleotides consisting of a 3′ random part used to prime complementary DNA synthesis and a 5′ defined tag seq...

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Autori principali: Rosseel, Toon, Van Borm, Steven, Vandenbussche, Frank, Hoffmann, Bernd, van den Berg, Thierry, Beer, Martin, Höper, Dirk
Natura: Artigo
Lingua:Inglês
Pubblicazione: Public Library of Science 2013
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Research Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3784409/
https://ncbi.nlm.nih.gov/pubmed/24086702
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0076144
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