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Construction of a thiamin sensor from the periplasmic thiamin binding protein
[Image: see text] This communication describes the development of a thiamin sensor based on the bacterial thiamin binding protein. A triple mutant (C48S, C50S, S62C) of TbpA was labeled on C62 with N-[2-(l-maleimidyl)ethyl]-7-(diethylamino)coumarin-3-carboxamide (MDCC). Thiamin binding to this prote...
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| Главные авторы: | , , , , |
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| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
2010
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3776480/ https://ncbi.nlm.nih.gov/pubmed/21125117 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c0cc04228e |
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