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Construction of a thiamin sensor from the periplasmic thiamin binding protein

[Image: see text] This communication describes the development of a thiamin sensor based on the bacterial thiamin binding protein. A triple mutant (C48S, C50S, S62C) of TbpA was labeled on C62 with N-[2-(l-maleimidyl)ethyl]-7-(diethylamino)coumarin-3-carboxamide (MDCC). Thiamin binding to this prote...

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Detalhes bibliográficos
Main Authors: Hanes, Jeremiah W., Chatterjee, Debashree, Soriano, Erika V., Ealick, Steven E., Begley, Tadhg P.
Formato: Artigo
Idioma:Inglês
Publicado em: 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3776480/
https://ncbi.nlm.nih.gov/pubmed/21125117
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c0cc04228e
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