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Urea-temperature phase diagrams capture the thermodynamics of denatured state expansion that accompany protein unfolding

We have analyzed the thermodynamic properties of the von Willebrand factor (VWF) A3 domain using urea-induced unfolding at variable temperature and thermal unfolding at variable urea concentrations to generate a phase diagram that quantitatively describes the equilibrium between native and denatured...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Tischer, Alexander, Auton, Matthew
Format: Artigo
Sprache:Inglês
Veröffentlicht: Blackwell Publishing Ltd 2013
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3776328/
https://ncbi.nlm.nih.gov/pubmed/23813497
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.2301
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