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Exploring the denatured state ensemble by single-molecule chemo-mechanical unfolding: the effect of force, temperature and urea
While it is widely appreciated that the denatured state of a protein is a heterogeneous conformational ensemble, there is still debate over how this ensemble changes with environmental conditions. Here, we use single-molecule chemo-mechanical unfolding, which combines force and urea using the optica...
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| Publicado no: | J Mol Biol |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6058696/ https://ncbi.nlm.nih.gov/pubmed/28782558 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2017.07.022 |
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