Agar Overlay Plaquing Technique for Pseudomonas aeruginosa in HeLa Monolayers

The solid agar overlay procedure used for viral plaquing was adapted to the study of Pseudomonas aeruginosa plaques in HeLa monolayers. After adsorption of the organism to the HeLa monolayers, an overlay consisting of 10% newborn calf serum (NBCS), 90% Eagles basal medium (EBM), and 1.5% agar contai...

Täydet tiedot

Bibliografiset tiedot

Julkaisussa:	Appl Microbiol
Päätekijät:	Wexler, Seymour, Ludovici, Peter P., Moore, Marion L.
Aineistotyyppi:	Artigo
Kieli:	Inglês
Julkaistu:	American Society for Microbiology (ASM) 1970
Aiheet:	Clinical Microbiology, Virology, and Immunology
Linkit:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC376930/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4991957/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/am.20.3.309-312.1970
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