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Agar Overlay Plaquing Technique for Pseudomonas aeruginosa in HeLa Monolayers

The solid agar overlay procedure used for viral plaquing was adapted to the study of Pseudomonas aeruginosa plaques in HeLa monolayers. After adsorption of the organism to the HeLa monolayers, an overlay consisting of 10% newborn calf serum (NBCS), 90% Eagles basal medium (EBM), and 1.5% agar contai...

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Bibliografische gegevens
Hoofdauteurs: Wexler, Seymour, Ludovici, Peter P., Moore, Marion L.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 1970
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC376930/
https://ncbi.nlm.nih.gov/pubmed/4991957
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