Agar Overlay Plaquing Technique for Pseudomonas aeruginosa in HeLa Monolayers

The solid agar overlay procedure used for viral plaquing was adapted to the study of Pseudomonas aeruginosa plaques in HeLa monolayers. After adsorption of the organism to the HeLa monolayers, an overlay consisting of 10% newborn calf serum (NBCS), 90% Eagles basal medium (EBM), and 1.5% agar contai...

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Dades bibliogràfiques

Publicat a:	Appl Microbiol
Autors principals:	Wexler, Seymour, Ludovici, Peter P., Moore, Marion L.
Format:	Artigo
Idioma:	Inglês
Publicat:	American Society for Microbiology (ASM) 1970
Matèries:	Clinical Microbiology, Virology, and Immunology
Accés en línia:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC376930/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4991957/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/am.20.3.309-312.1970
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