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Agar Overlay Plaquing Technique for Pseudomonas aeruginosa in HeLa Monolayers

The solid agar overlay procedure used for viral plaquing was adapted to the study of Pseudomonas aeruginosa plaques in HeLa monolayers. After adsorption of the organism to the HeLa monolayers, an overlay consisting of 10% newborn calf serum (NBCS), 90% Eagles basal medium (EBM), and 1.5% agar contai...

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Udgivet i:Appl Microbiol
Principais autores: Wexler, Seymour, Ludovici, Peter P., Moore, Marion L.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1970
Fag:
Clinical Microbiology, Virology, and Immunology
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC376930/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4991957/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/am.20.3.309-312.1970
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