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Rapid assessment of RNAi-mediated protein depletion by selected reaction monitoring (SRM) mass spectrometry
We describe the use of a targeted proteomics approach, Selected Reaction Monitoring (SRM) mass spectrometry, to detect and assess RNAi-mediated depletion or ‘knockdown’ of specific proteins from human cells and from Drosophila flies. This label-free approach does not require any specific reagents to...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3767422/ https://ncbi.nlm.nih.gov/pubmed/23713831 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/pr400067k |
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