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Characterization of a new oxidant-stable serine protease isolated by functional metagenomics

A novel serine protease gene, SBcas3.3, was identified by functional screening of a forest-soil metagenomic library on agar plates supplemented with AZCL-casein. Overproduction in Escherichia coli revealed that the enzyme is produced as a 770-amino-acid precursor which is processed to a mature prote...

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Hlavní autoři: Biver, Sophie, Portetelle, Daniel, Vandenbol, Micheline
Médium: Artigo
Jazyk:Inglês
Vydáno: Springer International Publishing 2013
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3765597/
https://ncbi.nlm.nih.gov/pubmed/24024096
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/2193-1801-2-410
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