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Identification and characterization of alkaline serine protease from goat skin surface metagenome
Metagenomic DNA isolated from goat skin surface was used to construct plasmid DNA library in Escherichia coli DH10B. Recombinant clones were screened for functional protease activity on skim milk agar plates. Upon screening 70,000 clones, a clone carrying recombinant plasmid pSP1 exhibited protease...
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| Autori principali: | , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Springer
2011
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3159910/ https://ncbi.nlm.nih.gov/pubmed/21906326 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/2191-0855-1-3 |
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