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Expression and Characterization of Coprothermobacter proteolyticus Alkaline Serine Protease
A putative protease gene (aprE) from the thermophilic bacterium Coprothermobacter proteolyticus was cloned and expressed in Bacillus subtilis. The enzyme was determined to be a serine protease based on inhibition by PMSF. Biochemical characterization demonstrated that the enzyme had optimal activity...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Hindawi Publishing Corporation
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3886229/ https://ncbi.nlm.nih.gov/pubmed/24453843 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1155/2013/396156 |
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