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Identification and characterization of alkaline serine protease from goat skin surface metagenome

Metagenomic DNA isolated from goat skin surface was used to construct plasmid DNA library in Escherichia coli DH10B. Recombinant clones were screened for functional protease activity on skim milk agar plates. Upon screening 70,000 clones, a clone carrying recombinant plasmid pSP1 exhibited protease...

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Bibliografske podrobnosti
Main Authors: Pushpam, Paul Lavanya, Rajesh, Thangamani, Gunasekaran, Paramasamy
Format: Artigo
Jezik:Inglês
Izdano: Springer 2011
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3159910/
https://ncbi.nlm.nih.gov/pubmed/21906326
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/2191-0855-1-3
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