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Purification and characterization of thiol dependent, oxidation-stable serine alkaline protease from thermophilic Bacillus sp.
Alkaline serine protease was purified to homogeneity from culture supernatant of a thermophilic, alkaliphilic Bacillus sp. by 80% ammonium sulphate precipitation followed by CM-cellulose and DEAE-cellulose ion exchange column chromatography. The enzyme was purified up to 16.5-fold with 6900 U/mg act...
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| Publicado no: | J Genet Eng Biotechnol |
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| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Academy of Scientific Research and Technology, Egypt
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6299798/ https://ncbi.nlm.nih.gov/pubmed/30647567 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jgeb.2015.01.002 |
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