Purification and characterization of thiol dependent, oxidation-stable serine alkaline protease from thermophilic Bacillus sp.

Alkaline serine protease was purified to homogeneity from culture supernatant of a thermophilic, alkaliphilic Bacillus sp. by 80% ammonium sulphate precipitation followed by CM-cellulose and DEAE-cellulose ion exchange column chromatography. The enzyme was purified up to 16.5-fold with 6900 U/mg act...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:J Genet Eng Biotechnol
Hauptverfasser: Kamran, Aysha, Ur Rehman, Haneef, Ul Qader, Shah Ali, Baloch, Abdul Hameed, Kamal, Mustafa
Format: Artigo
Sprache:Inglês
Veröffentlicht: Academy of Scientific Research and Technology, Egypt 2015
Schlagworte:
Microbial Biotechnology
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6299798/
https://ncbi.nlm.nih.gov/pubmed/30647567
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jgeb.2015.01.002
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