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Attenuated Protein Expression Vectors for Use in siRNA Rescue Experiments

Transient transfection of small interfering RNA (siRNA) provides a powerful approach for studying cellular protein functions, particularly when the target protein can be re-expressed from an exogenous siRNA-resistant construct in order to rescue the knockdown phenotype, confirm siRNA target specific...

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Detalhes bibliográficos
Main Authors: Morita, Eiji, Arii, Jun, Christensen, Devin, Votteler, Jörg, Sundquist, Wesley I.
Formato: Artigo
Idioma:Inglês
Publicado em: 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3759224/
https://ncbi.nlm.nih.gov/pubmed/22877307
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2144/000113909
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