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Attenuated Protein Expression Vectors for Use in siRNA Rescue Experiments
Transient transfection of small interfering RNA (siRNA) provides a powerful approach for studying cellular protein functions, particularly when the target protein can be re-expressed from an exogenous siRNA-resistant construct in order to rescue the knockdown phenotype, confirm siRNA target specific...
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Main Authors: | , , , , |
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Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
2012
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3759224/ https://ncbi.nlm.nih.gov/pubmed/22877307 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2144/000113909 |
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