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Imaging bacterial protein expression using genetically encoded sensors composed of RNA
We show that the difficulties in imaging the dynamics of protein expression in live bacterial cells can be overcome using fluorescent sensors based on Spinach, an RNA that activates the fluorescence of a small-molecule fluorophore. These RNAs selectively bind target proteins, and exhibit fluorescenc...
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| Main Authors: | , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2013
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3758421/ https://ncbi.nlm.nih.gov/pubmed/23872791 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.2568 |
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