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Imaging bacterial protein expression using genetically encoded sensors composed of RNA

We show that the difficulties in imaging the dynamics of protein expression in live bacterial cells can be overcome using fluorescent sensors based on Spinach, an RNA that activates the fluorescence of a small-molecule fluorophore. These RNAs selectively bind target proteins, and exhibit fluorescenc...

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Detalles Bibliográficos
Main Authors: Song, Wenjiao, Strack, Rita L., Jaffrey, Samie R.
Formato: Artigo
Idioma:Inglês
Publicado: 2013
Assuntos:
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC3758421/
https://ncbi.nlm.nih.gov/pubmed/23872791
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.2568
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