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In-gel imaging of RNA processing using Broccoli reveals optimal aptamer expression strategies
RNA aptamers can be expressed in cells to influence and image cellular processes. Aptamer folding is maintained by inserting the aptamers into highly structured RNA scaffolds. Here we show that commonly used RNA scaffolds exhibit unexpected instability and cleavage in bacterial and mammalian cells....
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| Publicado no: | Chem Biol |
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| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4441765/ https://ncbi.nlm.nih.gov/pubmed/26000751 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.chembiol.2015.04.018 |
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