Crystallographic analysis of an RNA polymerase σ-­subunit fragment complexed with −10 promoter element ssDNA: quadruplex formation as a possible tool for engineering crystal contacts in protein–ssDNA complexes

Structural studies of −10 promoter element recognition by domain 2 of the RNA polymerase σ subunit [Feklistov & Darst (2011 ▶), Cell, 147, 1257–1269] reveal an unusual crystal-packing arrangement dominated by G-quartets. The 3′-terminal GGG motif of the oligonucleotide used in crystallization pa...

Full description

Saved in:
Bibliographic Details
Main Authors: Feklistov, Andrey, Darst, Seth A.
Format: Artigo
Language:Inglês
Published: International Union of Crystallography 2013
Subjects:
Structural Communications
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3758139/
https://ncbi.nlm.nih.gov/pubmed/23989139
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309113020368
Tags: Add Tag
No Tags, Be the first to tag this record!

Similar Items