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Separation of long RNA by agarose-formaldehyde gel electrophoresis

We describe a method to facilitate electrophoretic separation of high molecular weight RNA species, such as ribosomal RNAs and their precursors, on agarose-formaldehyde gels. Two alternative “pK-matched” buffer systems were substituted for the traditionally used MOPS-based conductive media. The key...

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Hlavní autoři: Mansour, Farrah H., Pestov, Dimitri G.
Médium: Artigo
Jazyk:Inglês
Vydáno: 2013
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3755752/
https://ncbi.nlm.nih.gov/pubmed/23800830
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2013.06.008
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