Kinetics of drug selection systems in mouse embryonic stem cells

BACKGROUND: Stable expression of transgenes is an important technique to analyze gene function. Various drug resistance genes, such as neo, pac, hph, zeo, bsd, and hisD, have been equally used as selection markers to isolate a transfectant without considering their dose-dependent characters. RESULTS...

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Main Authors: Nakatake, Yuhki, Fujii, Setsuko, Masui, Shinji, Sugimoto, Toshimi, Torikai-Nishikawa, Satomi, Adachi, Kenjiro, Niwa, Hitoshi
Formato: Artigo
Idioma:Inglês
Publicado: BioMed Central 2013
Assuntos:
Methodology Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC3751694/
https://ncbi.nlm.nih.gov/pubmed/23919313
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-13-64
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