Kinetics of drug selection systems in mouse embryonic stem cells

BACKGROUND: Stable expression of transgenes is an important technique to analyze gene function. Various drug resistance genes, such as neo, pac, hph, zeo, bsd, and hisD, have been equally used as selection markers to isolate a transfectant without considering their dose-dependent characters. RESULTS...

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Bibliografske podrobnosti
Main Authors: Nakatake, Yuhki, Fujii, Setsuko, Masui, Shinji, Sugimoto, Toshimi, Torikai-Nishikawa, Satomi, Adachi, Kenjiro, Niwa, Hitoshi
Format: Artigo
Jezik:Inglês
Izdano: BioMed Central 2013
Teme:
Methodology Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3751694/
https://ncbi.nlm.nih.gov/pubmed/23919313
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-13-64
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