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Thinning of Large Biological Cells for Cryo-TEM Characterization by Cryo-FIB Milling
Focused ion beam milling at cryogenic temperatures (cryo-FIB) is a valuable tool that can be used to thin vitreous biological specimens for subsequent imaging and analysis in a cryo-transmission electron microscope (cryo-TEM) in their frozen-hydrated state. This technique offers the potential benefi...
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| Hauptverfasser: | , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
2012
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3748363/ https://ncbi.nlm.nih.gov/pubmed/22906009 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/j.1365-2818.2012.03635.x |
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