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Cryo-FIB specimen preparation for use in a cartridge-type cryo-TEM

Cryo-electron tomography (cryo-ET) is a well-established technique for studying 3D structural details of subcellular macromolecular complexes and organelles in their nearly native context in the cell. A primary limitation of the application of cryo-ET is the accessible specimen thickness, which is l...

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Detalhes bibliográficos
Publicado no:J Struct Biol
Main Authors: He, Jie, Hsieh, Chyongere, Wu, Yongping, Schmelzer, Thomas, Wang, Pan, Lin, Ying, Marko, Michael, Sui, Haixin
Formato: Artigo
Idioma:Inglês
Publicado em: 2017
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5555045/
https://ncbi.nlm.nih.gov/pubmed/28559166
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jsb.2017.05.011
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